When Jessica Drew was bitten by a poisonous spider as a child, her father saved her life by injecting her with an experimental "spider serum," which also granted her superhuman powers. As an adult, Jessica works as editor of Justice Magazine but when trouble arises, Jessica slips away to change into her secret identity of Spider-Woman.
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Joan Van Ark
Spider-Woman / Jessica Drew (voice) (16 episodes)
DT
Dick Tufeld
Narrator (voice) (16 episodes)
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Bruce Millar
Jeff Hunt (voice) (16 episodes)
BS
Bryan Scott
Billy (voice) (16 episodes)

Lou Krugman
Chief Cooper / General / Admiral Hubbard (voice) (16 episodes)
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Larry Carroll
Detective Miller (voice) (16 episodes)

Vic Perrin
Kuthu (voice) (16 episodes)

Paul Soles
Spider-man (voice) (2 episodes)